Background: Lower back pain (LBP) is a prevalent and debilitating condition among healthcare professionals and students, significantly affecting their quality of life, work productivity, and mental well-being. Non-specific LBP, which accounts for approximately 90% of all cases, is characterized by pain without an identifiable structural or neurological cause. This case report explores the diagnosis, management, and prevention strategies for non-specific LBP in healthcare professionals and students, with a focus on the role of proper body mechanics and ergonomics in mitigating the risk of injury. Case Presentation: A 38-year-old female registered nurse (RN) with 12 years of experience and a 24-year-old nursing student presented with non-specific LBP. Both cases were linked to improper body mechanics during patient care activities, such as lifting and transferring patients. The RN reported pain localized to the lower lumbar region, worsened by prolonged standing and bending, while the nursing student described similar symptoms following intensive clinical rotations. Both individuals were diagnosed with non-specific LBP based on clinical examination and imaging studies, which showed no structural abnormalities. A multimodal treatment approach was adopted, including pharmacological therapy (NSAIDs, muscle relaxants), physical therapy focusing on core and lumbar muscle strengthening, and education on ergonomic practices to improve posture and prevent further injury. Discussion: Non-specific LBP is a common issue among healthcare professionals and students, often exacerbated by physical strain and poor body mechanics during clinical tasks. The cases presented demonstrate the importance of early diagnosis and the integration of both pharmacological and non-pharmacological treatments. Pharmacological interventions, including NSAIDs and muscle relaxants, provide immediate relief, while physical therapy and ergonomic education address the root causes and help prevent recurrence. It is crucial to incorporate ergonomics and body mechanics training into healthcare curricula to reduce the incidence of musculoskeletal injuries and improve long-term health outcomes for both professionals and students. Conclusion: This case report underscores the effectiveness of a comprehensive, multimodal approach to managing non-specific LBP in healthcare professionals and students. Early intervention, proper ergonomic education, and a combination of pharmacological and rehabilitative treatments can significantly improve both pain management and functional recovery. Proactive measures, such as integrating ergonomics training into healthcare education, are essential for reducing the burden of LBP in this high-risk population.